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Stable Isotope Probing and Related Technologies

by Murrell, J. Colin
Published by : American Society for Microbiology (ASM) ISBN: 1555815375 Subject(s): Bioquímica | Biotecnología | Biochemistry, Biology & Biotechnology Year: 2011 List(s) this item appears in: Recursos de Bioquimica
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The concept of using stable isotopes to label and track microbes and metabolites of interest is a very recent development in microbial ecology. This comprehensive book is the first to present stable isotope probing (SIP) and its related technologies in a single volume. The book guides readers through the basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation, to optimal and future uses. Next, the book explores the important and emerging applications of SIP in environmental microbiology. The book concludes with explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. This book is recommended not only for students and researchers in environmental microbiology, but also for those who can apply these cutting-edge technologies to such fields as genomics, biotechnology, and medical microbiology.

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